Thinking Outside Of The Freezer: DNA And RNA Storage

Thinking Outside Of The Freezer: DNA And RNA Storage

Following the traditional ways, that have been a part of medicine for a long time, has its own perks. But moving ahead as the time paces forwards is also important. This can not only increase the precision of the ways that the tests are being conducted but also increase the quality of the results are produced at the end of the medical process. The reason that the old ways are still applicable is that they are producing the required results for the doctors and are also helping them in getting detailed knowledge about the patient. As much as it is important, the up gradation of the system is also very important.

Our blood is the main carrier of all the secret on-goings inside our system. It is made up of multiple elements that can be extracted to study the internal conditions of a human system.  As it is not possible to reach every part of the human system without using blades, therefore, the value of blood becomes tenfold as it travels across the body. If something is as relevant as blood, it is important to pay attention to the ways it is preserved at the medical facilities.

The two important elements of the blood that need to be preserved are namely DNA and RNA. As DNA carries the biological functions that help in maintaining the identification of the species, RNA is the acid that is produced as from DNA. One of the common ways to store both of these biological essentials is through the use of freezers. Keeping the blood cold has always been used a part of preservation technique that has been proved beneficial to the date and promises to continue doing its working in the future. Although there is no denying that this can cause a lot of electric consumption and if there is a way to reduce the excessive use of electricity, then it should be adopted as soon as possible.

High quality of these samples is necessary for accurate sequencing of DNA and RNA from a relevant individual. There are efforts being put in the tasks to store purified DNA in room temperature. Two ways can be adopted for this purpose:

  1. Using liquid nitrogen

The DNA sample can be preserved in a crystalline form to stop it from losing its properties.

  1. Dried DNA

Dehydration removes the water in the blood and stops the process of hydrolytic reactions thus preventing the strand breakage of DNA.

Using the process of multiple cycle freezing can result in the degradation of the DNA substance. If the blood is not pure anymore, then there is no chance that the results produced after working on it will be reliable too. Several drying techniques like spray drying, air drying, and Lyophilization are used as methods to fight blood contamination.

Along with DNA, RNA is also bothered by the wrong ways of preservation. It is speculated from the test results that the thaw cycles, cellular stress responses, and storage conditions have a great effect on the extracted RNA. As much as the method of Formalin-fixed paraffin-embedding is common, it still fails to make the RNA extraction difficult. That is because of fragmentation that happens as a result of the above-mentioned process. To prevent this, Flash freezing method is used that ends up giving better quality RNA. Despite being a superior method, it is not commonly used because of limited access to freezing facilities of that standard.

Several compounds are introduced in the market to aid in the preservation of RNA. They help by eliminating a need to freeze samples. This is made possible by providing an option to soak the small tissue pieces in a stabilizing solution. It makes the storage possible for normal degrees for a week, at 4C for 4 weeks and at -80C for 24 weeks (6 months).

All these ways can be a good trade-off for the traditional method of using freezers. Not only will they save money and prove out to be financial benefactor, but they are also necessary to have a greater quality of results that are produced by processing greater quality of DNA and RNA.

Here at ClinicalScoop we understand these needs and that is the reason our researchers and medical professionals are working tirelessly to solve the ambiguities that are present in the current medical scenarios.